Leica DMI6000 multifunction motorized inverted microscope (CMM)
This mulitfunction fully-motorized inverted microscope can capture 5Mpix color brightfield images (Leica DCF450 camera), as well as in DIC (Nomarski), and Polarization [Note: crossed polars only, this is not a full on POL microscope]. The sCMOS greyscale camera (Hamamatsu Flash 4.0) can capture 4Mpix images in 16bits at up to 30fps. The microscope has fluorescence cubes for dyes similar to DAPI, FITC/GFP, Rhodamine, and CY5. The microscope can also do phase contrast imaging, but the optical path is not currently configured for capturing phase images. The microscope has 2.5x, 5x, 10x, 20x, 40x dry objectives and 40x & 100x oil objectives, as well as a 1.0/1.6x optivar for intermediate magnifications. The stage can accomodate microscope slides, multiwell plates*, and small culture dishes (35mm, 70mm)*. We have a BiopTechs Delta-T live cell culture dish controller to allow for long-term live cell imaging at 37degrees C, but it has not been set up yet.
Capturing images is fairly easy and the Leica LAS-AF software is able to allow users to stich together multiple fields of view, creating a much larger image (color or greyscale cameras).
Installed on the microscope computer is a copy of HCImage 4.x, a 2D image analysis software that allows for semi-automated image analysis of a number of different parameters. Digital images captured from any appropriately calibrated source (e.g. confocal, TEM, SEM, and other microscopes or scanned images) can be analyzed by the software. The software has the capability to measure a large number of parameters related to size, shape, intensity, and position. In many instances the software can configured to be acquire measurements in a semi-automated mode. Basic statistical measurements are included in the software. Numerical data can be exported as Microsoft Excel™ spreadsheets. Most labs will need Mr. Cromey to develop a custom macro based on their research needs. Please consult with him before beginning the experiment.
ACCESS: Contact Mr. Cromey. LSN 429B has a keycode lock.
FEES: currently there are none (instrument is new and under warranty), but a small fee is under consideration for July 1, 2016.
* low mag images through plastic dish bottoms are possible, but higher magnification image capture or DIC/POL images require the use of a coverslip thickness (#1.5, 0.170mm) glass bottom on the dish.