Leica SP5-II confocal microscope (UACC)

Leica SP5-II confocal microscope (UACC)
Required safety certifications?: 
Laser safety
Can non-UA users access this equipment?: 
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Salmon Cancer Center Building [222.01]
Room #: 
Technical expert(s): 

The instrument has the following features:

  • Excitation wavelengths of 405, 458, 476, 488, 514, 543, and 633nm
  • Objective lenses: 5x/0.15NA PL Fluotar, 10x0.4 PL Apo, 20x/0.7NA Plan Apo, 20x/0.7NA Plan Apo multi-IMM (oil, water, glycerin), 40x/1.25NA PL Apo (oil), 63x/1.4 NA PL Apo (oil)
  • Inverted microscope stand with environmental control chamber for live cell imaging (multi-well plates, 35-70mm dishes with coverslip thickness bottom).
  • Capable of capturing transmitted light images (DIC optics are currently not available)
  • Maximum image size available is 8192 x 8192 pixels.
  • "Stacks" of images can be acquired in Z (focus depth), Lambda (wavelength scan) or T (time series).
  • FRET (fluorescence resonance energy transfer), FRAP (fluorescence recovery after photobleaching), Ratio imaging (e.g., pH or ion-sensitive dyes) .
  • The instrument includes a tandem scan head with resonant scanner that allows 8kHz scanning speed. The resonant scanner can provide imaging speeds of up to 28 512 x 512 images/second.
  • Using the motorized XY stage, multiple images can be captured to create large multi-image montages or to capture data from specific points in a sample in a time course experiment.
  • The instrument's prism and slit technology allows tremendous flexibility in designing custom filter settings for any experiment.