Nikon Total Internal Reflection (TIRF) Microscope (Chem & Biochem)

Nikon TIRF Microscope
Required safety certifications?: 
Laser safety
Can non-UA users access this equipment?: 
Yes
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Building: 
Chemical Sciences [44]
Room #: 
135
Technical expert(s): 
Capabilities: 

Surface specific fluorescence imaging of samples is generated using evanescent excitation, penetration depth of ~100 nm in the Z direction, on the TIRF Microscope. The Nikon Ti-E TIRF inverted microscope is equipped with 3 excitation sources including 6 excitation wavelengths (408, 457, 477, 488, 514, and 561 nm), an imaging CCD detector (Andor DU 897), and a spectral detection system (Princeton Instruments). The Ti-E is equipped with a perfect focus motorized nosepiece, an additional emission filter wheel, a CFI APO 60x Oil TIRF objective (NA=1.49), and a CFI APO 100x Oil TIRF objective (NA = 1.49). This microscope is capable of detecting single molecule fluorescence.

Imaging Modes: Epi-Fluorescence, Objective based Total Internal Reflection Imaging, and Spectral Detector

Fees as of February 1st, 2015:  Internal $19/hour; Internal Assisted $49/hour; External Assisted $122/hour

PUBLICATION ACKNOWLEDGEMENT: All TIRF images and data were collected in the W.M. Keck Center for Nano-Scale Imaging in the Department of Chemistry and Biochemistry at the University of Arizona with funding from the W.M. Keck Foundation Grant.