Olympus IMT-2 (CMM)

Olympus IMT-2 (CMM)
Can non-UA users access this equipment?: 
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Life Sciences North [221]
Room #: 
Technical expert(s): 

The Olympus IMT-2 inverted microscope is equipped with a digital camera that can capture 1280x1024, 10 bit (1024 intensity or grayscale values) images of brightfield, phase contrast, and DIC imaging modes. The microscope has motorized controllers for shutters, and x-y-z stage control. The automated stage has holders for microscope slides, 35mm culture dishes and multiwell well plates (note: DIC imaging uses polarization and cannot be performed through plastic). A BiopTechs Delta T3 system for keeping 35mm culture dishes at 37°C enables the system to be able to capture time-lapse images of cultured cells over periods of several hours. A copy of Microsoft Excel™ is available on the computer to assist in transferring the numerical data.

Image Analysis & Measurement Capabilities:
The workstation uses the SimplePCI software (C•Imaging Systems, by Hammamatsu, Inc.). Digital images captured from any appropriately calibrated source (e.g. confocal, TEM, SEM, and other microscopes or scanned images) can be analyzed by the software. The software has the capability to measure a large number of parameters related to size, shape, intensity and position. In many instances the software can configured to be acquire measurements in a semi-automated mode. Basic statistical measurements are included in the software. Numerical data can be exported as Microsoft Excel™ spreadsheets.

The software has been used to measure/capture:

  • The distance traveled by cultured cells over time (time lapse)
  • The length of dendrites (confocal images)
  • Calculate pH based on using color ratios from SNARF dyes (Zeiss confocal images)
  • Point counting in lung tissue (light microscope images)
  • Image montages of whole cross-sections that were later used for 3D reconstruction or poster presentations (light microscope images)
  • Create time-lapse movies for presentations (light microscope images)
  • Count fluorescently stained nuclei to measure migration in a matrix (fluorescence microscope images)
  • Measure sizes of intestinal villi in histologic samples (light microscope images)
  • Measure the area of lipid droplets found in Oil Red O stained histologic samples (light microscope images)
  • 3D rotations of multi-level/Z-stack images (light microscope images)
  • Automatically count the number of cells that crossed the membrane in transwell plates (24 well plates).
  • Analysis of changes in melanized skin moles to assess the success of cancer treatment (macro photography).