2017 Optical Microscopy and Imaging in the Biomedical Sciences (OMIBS) course

Announcing the 2017 Optical Microscopy and Imaging in the Biomedical Sciences (OMIBS) course, offered by the Marine Biology Laboratory, Woods Hole, MA. This outstanding light microscopy course was established in 1978 and has been offered annually since then.


Course dates: September 6 - September 16, 2017

Application Deadline: Applications accepted through June 13, 2017

Where: In the newly renovated and modernized facilities in the Loeb Bldg at the Marine Biology Laboratory, Woods Hole, MA

What: Imaging fundamentals to state of the art, multi-dimensional wide field, fluorescence, confocal, multiphoton, multispectral, deconvolution, super-resolution, and light-sheet microscopy systems.

How: Lectures, hands on exercises, problem sets, demonstrations and discussions, specifically designed to drive home the principles and applications of modern microscopy. Students will learn to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce digital records for documentation and analysis. In the problem sets, small groups of students will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopy and imaging skills.

Equipment: Microscopes, Cameras, and Software from more than 19 World Renowned Companies.

For Whom: Designed primarily for research scientists, postdoctoral trainees and advanced graduate students in animal, plant, and medical sciences. Facility Directors and non-biologists seeking a comprehensive introduction to microscopy and digital imaging in the biomedical sciences will also benefit greatly from this course. There are no specific prerequisites, but an understanding of the basic principles of optics and imaging is desirable. The course is limited to 26 students.

Specimens: Live and fixed specimens will be provided for use in laboratory exercises and demonstrations. However, students are encouraged to bring their own biological (cell cultures, prepared slides, etc.) and material specimens to use in exercises throughout the course, where appropriate. Cell culture facilities are available for student use. Students also are encouraged to make appointments with our experienced Commercial Faculty outside of scheduled classes to acquire data from their specimens using the state of the art equipment available. Students are highly encouraged to discuss their individual research problems with both the academic and commercial faculty.

Topics To Be Covered Include:

  • Principles of microscope design and image formation, image resolution, and image contrast.
  • Bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy
  • Cameras, digital imaging, signal to noise ratio, recording, enhancement, processing and analysis
  • Advanced fluorescence techniques (e.g. FRAP, FLIP, speckle imaging, FRET, FLIM, fluorescence polarization, photoactivation, photoswitching, fluorescence correlation spectroscopy, multi-spectral imaging, filter cubes, fluorescent dyes/proteins)
  • Confocal scanning microscopy (LSM and spinning disc), multiphoton excitation fluorescence microscopy and structured illumination systems
  • Image deconvolution, digital image restoration
  • 3D image acquisition, display, and analysis
  • Live cell imaging
  • Super-resolution techniques (STED, PALM, STORM, SIM)
  • Light-sheet microscopy

Tuition: $3500 (Includes text, handouts, supplies)
Room and board: $702.50
Financial Assistance is Available!!!

Course Directors:

Dr. Robert Hard
Dept. Pathology and Anatomical Sciences, School of Medicine and Biomedical Sciences, University at Buffalo
Phone: [716] 829-3521
Email: hard@acsu.buffalo.edu

Dr. Hari Shroff
NIH/NIBIB, Section on High Resolution Optical Imaging, National Institutes of Health
Phone: [301] 435-1995
Email: hari.shroff@nih.gov

Admission application and information:

Carol Hamel, Admissions Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543-1015
(508) 289-7401
Email: admissions@mbl.edu
WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat)

See Also: http://www.mbl.edu/education/courses/optical-microscopy-imaging/

 

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