Digital Light Sheet Imaging Seminar (Leica)

Presenter: Kristofer Fertig, Senior Confocal Sales and Applications Specialist, Leica Microsystems
Time: 2-3pm
Date: Monday, April 16, 2018
Location: Medical Research Building, room 102
Other: Light refreshments will be provided.
Light sheet fluorescence microscopy (LSFM) uses a thin plane of excitation light that shines into a sample at 90 degrees to the imaging axis. Since the fluorescent molecules in the sample are only excited by the thin sheet of light, images can be captured without the problems of out-of-focus fluorescence above or below the plane of interest. By rotating the sample, in-focus images can be captured and reconstructed for the entire volume of the sample. Because LSFM can reduce the overall light exposure to the sample, many labs have successfully used the technique for demonstrating changes in live cells or embryonic development over time. The Nature Methods journal named Light Sheet Microscopy the 2014 method of the year. Leica uses a novel add-on to their SP8 confocal microscopes to perform lightsheet microscopy.
As with any microscopic technique there are often trade-offs. It can be challenging to obtain the best resolution possible (e.g., something close to confocal resolution) with large samples.
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