Off-campus Microscopy Courses (summer & fall 2018)

Biosensors and Force Measurements in Living Cells (10th Annual)

Date: May 15-18, 2018
Deadline: unknown
Location: University of North Carolina - Chapel Hill, NC, USA
Contact: Cassandra Houston, houstonc@physics.unc.edu or (919) 962-4057
 
Course content:
  • Theory and use of the application of forces to cells, and the measurement of responses to forces, using fluorescence-based biosensors, a combined Atomic Force Microscopy (AFM) – optical microscope system combined with vertical light sheet, and UNC’s magnetic tweezers system
  • Tuesday/Wednesday morning lectures: overview of mechanisms of force generation in cells, and analysis of applied forces at the molecular and cellular level; optical microscopy using FRET based biosensors and light activated signaling molecules
  • Tues/Wed/Thursday afternoon labs: hands on experiments using AFM, total internal reflection fluorescence (TIRF) microscopy, and magnetic force systems on live biological samples
  • Thursday/Friday Morning Lectures: Image Analysis using ImageJ/FIJI for quantifying microscope data (presented by Teng-Leong Chew, HHMI Janelia Research Campus)

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Montreal Light Microscopy Course 2018 - Frontiers: 3D Imaging: Dead or Alive

Date: July 16-20, 2018
Deadline: May 4, 2018
Location: Montreal, CA
 
The July 2018 MLMC is designed to provide a week of advanced instruction in optical microscopy. Core facility staff and managers, graduate students, post doctoral fellows, PIs, and research scientists are all welcome to attend.
 
Topics
  • Imaging Fixed Samples
  • Live Cell Imaging
  • Photo-Manipulation
  • Measuring Cellular Dynamics
  • Light Sheet Microscopy
  • Multi-Photon Microscopy
  • Laser Scanning Confocal
  • Spinning Disk Confocal
  • Multi-Dimensional Imaging
  • Image Processing and Analysis
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Biological TEM Workshop

Date: July 25-27, 2018
Deadline: July 20, 2018
Location: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Contact: John Shields, jpshield@uga.edu 
 
This intensive, three-day workshop will provide a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and *hands-on* training led by GEM staff.
 
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2018 "Optical Microscopy and Imaging in the Biomedical Sciences" (OMIBS) course

Date: Aug 20-30, 2018.
Deadline: May 15, 2018
Location: Marine Biological Laboratory, Woods Hole, MA, USA.
Course Directors: Dr. Alison North (Rockefeller University) and Dr. Joerg Bewersdorf (Yale University)
 
The course takes participants all the way from the fundamentals of optical microscopy through to state-of-the-art, multi-dimensional wide field fluorescence, confocal, multiphoton, multispectral, deconvolution, super-resolution and light-sheet microscopy systems.Participants will learn through lectures, hands-on exercises, problem sets, demonstrations and discussions, specifically designed to drive home the principles and applications of modern microscopy.
 
In this year's course a new emphasis will also be placed on *sample preparation*, including fluorophore selection, tissue clearing and expansion microscopy. Students will learn to obtain and interpret microscope images of high quality, to assess data for common errors and artifacts, and to perform quantitative optical measurements. In the problem sets, small groups of students will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopy and imaging skills.
 
The course is designed primarily for research scientists, postdoctoral trainees and graduate students in the biological sciences. Facility Directors and non-biologists seeking a comprehensive introduction to microscopy and digital imaging in the biomedical sciences will also benefit greatly from this course.
 
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Next-Generation Transmission Electron Microscopy (NexTEM) Workshop

Beyond Current Limits of Resolution, Environments, and Data Analysis

Date: October 8-10, 2018
Location: Discovery Hall, Pacific Northwest National Laboratory, Richland, WA Workshop Organizers: Steven Spurgeon, PNNL | Mitra Taheri, Drexel University
 
Pacific Northwest National Laboratory is pleased to host the inaugural Next-Generation Transmission Electron Microscopy (NexTEM) workshop. This event will include three full days of invited and contributed sessions on topics including high-resolution imaging and spectroscopy, in situ / operando microscopy in extreme environments, as well as computational methods for data analysis. NexTEM will bring together experts in cutting-edge imaging methods, technique development, and data analytics to identify emerging pathways to more efficient and insightful materials analysis in real-world conditions.
 
Topics
 
High-Resolution Imaging and Spectroscopy – New analytical transmission electron microscopy instrumentation and methods to characterize nanoscale systems.
  • Measurement of local atomic structure, chemistry, and composition with high sensitivity and precision.
  • Correlative STEM, EDS, and EELS imaging to probe complex defects, crystals, and interfaces.
  • Methods to conduct high-resolution imaging and spectroscopy of beam-sensitive samples.
  • Novel detectors for improved high-speed imaging and spectroscopy.
 
In Situ / Operando Microscopy and Extreme Environments – Advances in S/TEM methods and instrumentation to capture the dynamics of complex materials systems, including alloys, thin films, nanoparticles, and liquids.
  • Investigation of materials under stimulus across a range of sample environments and temperatures.
 
Computational Methods for Data Analysis
  • Software to improve data collection quality, accuracy, and acquisition rates.
  • Methods to enhance the signal-to-noise of low-dose images and spectroscopy, including compressive sensing and multivariate statistical analysis.
  • Machine learning approaches for high-throughput data processing and feature detection.
  • Image simulation tools to aid the interpretation of experimental images and spectroscopy.
 
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