Fluorescence

Kurt Gustin

Dr. Gustin provides training on the Zeiss Axio Imager upright fluourescent microscope located in ABC1 on the COM-Phx campus. His laboratory has experience with fluorescent imaging, acquiring z-stacks, deconvolution and image processing and analysis. 

Digital Light Sheet Imaging Seminar (Leica)

Presenter: Kristofer Fertig, Senior Confocal Sales and Applications Specialist, Leica Microsystems
Time: 2-3pm
Date: Monday, April 16, 2018
Location: Medical Research Building, room 102
Other: Light refreshments will be provided.
 
Light sheet fluorescence microscopy (LSFM) uses a thin plane of excitation light that shines into a sample at 90 degrees to the imaging axis.

Zeiss LSM 710 Confocal (COM-PHX)

Shenfeng Qiu

Dr. Shenfeng Qiu also trains new users for the Phoenix Biomedical Campus' Biomedical Imaging Core facility (confocal, and two photon microscope). His group has utilized confocal microscopy for multiple fluorophore acquisition, and also has performed in vivo imaging in mouse brain.

Light Sheet Microscopy (lunch seminar)

Nature Methods journal named Light Sheet Microscopy the 2014 method of the year. Light sheet microscopy illuminates the sample in a single plane while collecting the images in the perpendicular plane using low light level, high speed cameras. The sample can be optically sectioned and imaged in 3D by moving the sample through the various planes. This method is a faster method of collecting optical sections while reducing the amount of photo-damage to the sample. This allows for imaging developing embryos, imaging high speed calcium signals or rapidly collecting large data sets.

Super-Resolution Imaging - The ELYRA SR-SIM System and Discussion of Other Superresolution Techniques (seminar)

Installation was completed in March 2016 of the campus’ new superresolution microscope, the Zeiss Elyra S.1. This instrument is capable of twice the resolving power of existing confocal and deconvolution fluorescence microscopes.

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