3i Spinning Disk Confocal (Chem & Biochem)

Spinning Disk Microscope
Required safety certifications?: 
Laser safety
Can non-UA users access this equipment?: 
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Biological Sciences West [88]
Room #: 
Technical expert(s): 

The Spinning Disk Confocal on an inverted microscope base with DIC, transmitted light, and epi-Fluorescence.  The system is equipped with two fluorescence laser excitation sources at 488 nm and 561 nm.  The detector is a Photometrics Evolve CCD detector with optional Dual View attachment. Definite Focus is available for maintaining focus during timelapse experiments.  A 150 um piezoelectric Z stage and Eppendorf Transferman micromanipulator are mounted on the system.  A Plan-Neofluar 20X/0.5 NA air, Plan-Neofluar 40X/1.3 NA oil, and a Plan-Apochromat 63X/1.4 NA oil objectives are available.  An OKO Lab enviromental enclosure is on the microscope for temperature, gas (CO2, O2 and air/N2), and humidity control during experiments.

Imaging Modes: DIC, Transmitted Light, Epi-Fluorescence, Confocal Fluorescence and FRET

Fees as of February 1st, 2015:  Internal $10/hour; Internal Assisted $40/hour; External Assisted $78/hour

PUBLICATION ACKNOWLEDGEMENT: All Confocal images and data were collected in the W.M. Keck Center for Nano-Scale Imaging in the Department of Chemistry and Biochemistry at the University of Arizona.  This instrument purchase was partially supported by Arizona Technology and Research Initiative Fund (A.R.S.§15-1648).