3i Spinning Disk Confocal (Chem & Biochem)

The Spinning Disk Confocal on an inverted microscope base with DIC, transmitted light, and epi-Fluorescence. The system is equipped with two fluorescence laser excitation sources at 488 nm and 561 nm. The detector is a Photometrics Evolve CCD detector with optional Dual View attachment. Definite Focus is available for maintaining focus during timelapse experiments. A 150 um piezoelectric Z stage and Eppendorf Transferman micromanipulator are mounted on the system. A Plan-Neofluar 20X/0.5 NA air, Plan-Neofluar 40X/1.3 NA oil, and a Plan-Apochromat 63X/1.4 NA oil objectives are available. An OKO Lab enviromental enclosure is on the microscope for temperature, gas (CO2, O2 and air/N2), and humidity control during experiments.
Imaging Modes: DIC, Transmitted Light, Epi-Fluorescence, Confocal Fluorescence and FRET
Fees as of February 1st, 2015: Internal $10/hour; Internal Assisted $40/hour; External Assisted $78/hour
PUBLICATION ACKNOWLEDGEMENT: All Confocal images and data were collected in the W.M. Keck Center for Nano-Scale Imaging in the Department of Chemistry and Biochemistry at the University of Arizona. This instrument purchase was partially supported by Arizona Technology and Research Initiative Fund (A.R.S.§15-1648).