Leica DMI6000 multifunction motorized inverted microscope with two cameras

Leica DMI6000 multifunction motorized inverted microscope
Can non-UA users access this equipment?: 
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Life Sciences North [221]
Room #: 
Technical expert(s): 

COVID-19 Policies for all users (effective June 1, 2020).

This mulitfunction fully-motorized inverted microscope can capture both 24bit color transmitted light images, and 16bit epi-fluorescence images.

The microscope includes a 5Mpix camera for color brightfield images (Leica DCF450), as well as in DIC (Nomarski), and Polarization [Note: crossed polars only, this is not a full on POL microscope]. The sCMOS greyscale camera (Hamamatsu Flash 4.0) can capture 4Mpix images in 16bits at up to 30fps. The microscope has fluorescence cubes for dyes similar to DAPI, FITC/GFP, Rhodamine, and CY5. The microscope can also do phase contrast imaging, but the optical path is not currently configured for capturing phase images. The microscope has 2.5x, 5x, 10x, 20x, 40x dry objectives and 40x & 100x oil objectives, as well as a 1.0/1.6x optivar for intermediate magnifications. The stage can accommodate microscope slides, multiwell plates*, and small culture dishes (35mm, 70mm)*. We have a BiopTechs Delta-T live cell culture dish controller to allow for long-term live cell imaging at 37 degrees C.

Capturing images is fairly easy and the Leica LAS-X software is able to allow users to stitch together multiple fields of view, creating a much larger image (color or greyscale cameras).

Installed on the microscope computer is a copy of HCImage 4.x, a 2D image analysis software that allows for semi-automated image analysis of a number of different parameters. Digital images captured from any appropriately calibrated source (e.g. confocal, TEM, SEM, and other microscopes or scanned images) can be analyzed by the software. The software has the capability to measure a large number of parameters related to size, shape, intensity, and position. In many instances the software can configured to be acquire measurements in a semi-automated mode. Basic statistical measurements are included in the software. Numerical data can be exported as Microsoft Excel™ spreadsheets. Most labs will need Mr. Cromey to develop a custom macro based on their research needs. Please consult with him before beginning the experiment.

ACCESS: Contact Mr. Cromey. LSN 429B has a keycode lock.

FEES: $10/hr (as of 1/2/2017; NOTE: training does not incur any additional fees).

* low mag images through plastic dish bottoms are possible, but higher magnification image capture or DIC/POL images require the use of a coverslip thickness (#1.5, 0.170mm) glass bottom on the dish.

Acknowledging the Core: “The Leica DMI6000B microscope is part of the Imaging Cores – Life Sciences North, which is overseen by the University of Arizona’s Arizona Research Labs (purchase of this instrument was supported by the Arizona Technology and Research Initiative Fund, A.R.S.§15-1648).”
If appropriate, acknowledging Doug Cromey: “The authors would like to acknowledge the assistance of Douglas Cromey, MS, manager for optical microscopes in the Imaging Cores – Life Sciences North.”

Technique-related tags (user generated):