Zeiss ELYRA S1 (SR-SIM) Super Resolution Microscope

Zeiss Elyra S.1 SR-SIM microscope
Can non-UA users access this equipment?: 
Yes
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Building: 
Life Sciences North [221]
Room #: 
410B
Technical expert(s): 
Capabilities: 

The Zeiss Elyra S.1 uses the technique of structured illumination (SR-SIM) to achieve resolutions two times better than that of a confocal, deconvolution or standard fluorescence microscope. Resolutions in XY of 110nm and in Z of 300nm are possible (resolution is wavelength and sample-preparation dependent), allowing users to image a volume in the sample that is 1/8 that of previous techniques.

The good news is that this super-resolution technique is a not-too-difficult jump from the dyes and sample prep techniques used for a confocal or deconvolution microscope. The Elyra S.1 can image four fluorescent colors, roughly corresponding to DAPI, FITC/GFP, Rhodamine/RFP, and CY5 (see below). SR-SIM can image to depths of 10-15 microns into a sample. Note: SR-SIM does not work well (or at all) with dimly fluorescent samples or samples that photo-bleach.

Image Gallery: http://microscopy.arizona.edu/sim-image-gallery

FEES (UA users, external users please contact us):

  • Routine use - $45/hr
  • Assisted Use/Training - $90/hr

Information about Structured Illumination Microscopy (SIM) and important sample preparation tips.

The Elyra S.1 has the following microscope objectives:

  • Plan-Neofluar 10x/0.30
  • Plan-APO 40x/1.4 Oil
  • Plan-Apochromat 63x/1.40 Oil
  • Plan-Apochromat 63x/1.30 Oil/Water/Glycerine KORR
  • Alpha Plan-APO 100x/1.46 Oil

The laser lines are 405, 488, 561, and 642nm, with the filter specs of:

  • 405nm excitation (MBS 405 + EF BP 420-480 / LP 750)
  • 488nm excitation (MBS 488 + EF BP 495-550 / LP 750)
  • 561nm excitation (MBS 561 + EF BP 570-620 / LP 750)
  • 642nm excitation (MBS 642 + EF LP 655)

 

Location: