Zeiss LSM880 NLO upright multiphoton/confocal microscope

Required safety certifications?: 
Laser safety
Can non-UA users access this equipment?: 
Yes
Who can use this equipment?: 
Trained users (inquire with the facility regarding training)
Building: 
Marley Building [107]
Room #: 
101
Technical expert(s): 
Capabilities: 

The Zeiss LSM 880 NLO upright multiphoton/confocal microscope includes a 34-channel detector for spectral information and additional non-descanned GAsP detectors. The system includes 7 visible spectrum laser lines (405, 458, 488, 514, 561, 633nm) in addition to the software tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm). The system includes additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP & FCS and control for the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.

The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

Objective lenses (with matching DIC prisms) include:

  • N-Achroplan 5x/0.15 (WD=12.0mm)
  • Plan-Apochromat 10x/0.45 (WD=2.0mm)
  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • W Plan-Apochromat 20x/1.0 Water
  • C-ACHROPLAN 32x/0.85 W Korr Water
  • EC Plan-Apochromat 40x/1.3 Oil
  • C Planapo 40x/1.3 Oil
  • LD C-Apochromat 40x/1.1 W Corr Water
  • Plan-Apochromat 63x/1.40 Oil
  • W Plan-Apo 63x/1.0 Water (WD=2.1mm)
  • Plan-Apochromat 100x/1.4 Oil
  • Additional lenses associated with the adjacent Zeiss LSM880 confocal may be available for this instrument (contact the facility manager to discuss this)

FEES (UA users, external users please contact us): 

  • Routine use - $45/hr
  • Assisted Use/Training - $115/hr
Location: