Zeiss LSM880 NLO upright multiphoton/confocal microscope
The Zeiss LSM 880 NLO upright multiphoton/confocal microscope includes a 34-channel detector for spectral information and additional non-descanned GAsP detectors. The system includes 7 visible spectrum laser lines (405, 458, 488, 514, 561, 633nm) in addition to the software tunable Spectra Physics Mai Tai HP DeepSee laser (690-1040 nm). The system includes additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP & FCS and control for the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.
The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.
Objective lenses (with matching DIC prisms) include:
- N-Achroplan 5x/0.15 (WD=12.0mm)
- Plan-Apochromat 10x/0.45 (WD=2.0mm)
- Plan-Apochromat 20x/0.8 (WD=0.55mm)
- W Plan-Apochromat 20x/1.0 Water
- C-ACHROPLAN 32x/0.85 W Korr Water
- EC Plan-Apochromat 40x/1.3 Oil
- C Planapo 40x/1.3 Oil
- LD C-Apochromat 40x/1.1 W Corr Water
- Plan-Apochromat 63x/1.40 Oil
- W Plan-Apo 63x/1.0 Water (WD=2.1mm)
- Plan-Apochromat 100x/1.4 Oil
- Additional lenses associated with the adjacent Zeiss LSM880 confocal may be available for this instrument (contact the facility manager to discuss this)
FEES (UA users, external users please contact us):
- Routine use - $45/hr
- Assisted Use/Training - $115/hr