A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

Advanced microscopy bootcamp (July 2024) - Ashburn VA

The Janelia Advanced Imaging Center (AIC) will offer a 12-day intensive microscopy bootcamp– Optical Imaging Across Biological Length Scales–in 2024. This course will be held at Janelia Research Campus July 28-August 9, 2024. There is no application fee and Janelia will cover the costs of lodging and food for all participants. Applications are due by Feb 15, 2024.

Participants will receive didactic instruction and hands-on experience with localization super-resolution microscopy, stimulated emission-depletion (STED) super-resolution microscopy, enhanced super-resolution methods such as structured illumination microscopy and AiryScan, expansion microscopy, and various light sheet microscopy methods. Participatory lab sessions will be conducted with biological samples from single cells to various model organisms, on commercial instruments as well as pre-commercial instruments developed at Janelia, including iPALM, lattice light sheet, MOSAIC, and SiMView. The course will put a strong emphasis on hypothesis-driven experimental design and image processing. Participants will also receive in-depth training in open-source software and methods for handling big data, as well as ethical reporting of microscopy data. The bootcamp will feature plenary lectures by Janelia senior group leaders Luke Lavis, Jennifer Lippincott-Schwartz, Hari Shroff, and Ron Vale.

Instruments involved in the Bootcamp Course include:

  • Interferometric PALM (Hess, Janelia)
  • Lattice light sheet microscope (Betzig, Janelia) 
  • MOSAIC (Betzig, Janelia) 
  • SiMView light sheet microscope (Keller, Janelia) 
  • MuVi SPIM LS/CS/PM (Bruker) 
  • Lightsheet 7 (Zeiss) 
  • Lattice Lightsheet 7 (Zeiss) 
  • Elyra 7 Lattice SIM (Zeiss) 
  • Expert Line STED (Abberior) 
  • LSM 980 with AiryScan confocal microscope (Zeiss)
  • CSU-W1 SoRa spinning disk confocal microscope with TIRF (Nikon)

Applications are open to anyone interested in understanding the full spectrum of optical imaging capabilities. Graduate students, postdocs, independent scientists, faculty, and core facility managers from non-profit organizations are encouraged to apply. To be considered, applicants must justify their need to learn multi-scale imaging on various modalities and expand on prior imaging experience. Applicants must explain their motivation for taking the course and how it will help their work. The course is designed to provide advanced training in optical microscopy. Prior microscopy experience is preferred.

More information and the application form can be found here: https://www.aicjanelia.org/imagingacrosslengthscales