A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

Analytical and Quantitative Light Microscopy course (April 2024) - Woods Hole, MA

Registration is now open for the 2024 Analytical and Quantitative Light Microscopy, to be held at the Marine Biological Laboratory, Woods Hole, MA, USA.

AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and the application of digital methods to understand the subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field microscopes, confocal microscopes, and new emerging technologies. Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab, led by leaders in the fields of microscopy and cell biology. It's intense, it's hardcore, and it's really fun!

Location: Marine Biological Laboratory, Woods Hole, MA, USA.
Dates: April 24th-May 3rd, 2024
Deadline: Applications are due *December 27th, 2023*
Register at: http://www.mbl.edu/aqlm  Financial assistance is available.

Laboratory exercises, demonstrations, and discussions include:

  • geometrical and physical optics of microscope image formation including Abbe’s theory of the microscope and Fourier optics;
  • label free imaging: phase contrast, polarization, and interference microscopy
  • epifluorescence and confocal microscopy, quantification of fluorescence, and fluorescent proteins;
  • principles and application of quantitative 3D digital imaging
  • digital image processing, automated analysis, and machine learning;
  • live cell imaging and FRET based biosensors
  • specialized methods including TIRF, FLIM and light-sheet;
  • Super-resolution SIM and SMLM
  • This year, we are also adding a Labeling Techniques Section!

Course Directors:

  • Peter Kner (University of Georgia)
  • Alexa Mattheyses (University of Alabama - Birmingham) Course Laboratory Director:
  • Gary Laevsky (Princeton University)