Analytical and Quantitative Light Microscopy (Woods Hole, MA) April 2025
We are excited to announce this year's run of Analytical and Quantitative Light Microscopy, held at the Marine Biological Laboratory, Woods Hole, MA, USA.
The course will run April 30th-May 9th, 2025. Applications are due *December 16th, 2024*. Apply through the course web site at http://www.mbl.edu/aqlm Financial assistance is available. *The application portal is open NOW!*
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and the application of digital methods to understand the subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field microscopes, confocal microscopes, and new emerging technologies.
Laboratory exercises, demonstrations, and discussions include:
- geometrical and physical optics of microscope image formation including Abbe’s theory of the microscope and Fourier optics
- label free imaging: phase contrast, polarization, and interference microscopy
- epifluorescence and confocal microscopy, quantification of fluorescence, and fluorescent proteins
- principles and application of quantitative 3D digital imaging
- digital image processing, automated analysis, and machine learning
- live cell imaging and FRET based biosensors
- specialized methods including TIRF, FLIM and light-sheet
- Super-resolution STED and SMLM
*This year, we are also adding a Labeling Techniques Section!*
Within the course, we often refer to AQLM as "Microscopy Boot Camp". We cover every topic with a lecture and an in-depth hands-on lab, led by leaders in the fields of microscopy and cell biology. It's intense, it's hardcore, and it's really fun! Join us for AQLM (or recommend one of your lab members or core facility users).
AQLM 2025 Faculty
- Alexa Mattheyses, University of Alabama, Birmingham
- Peter Kner, University of Georgia
- Emily Bartle, University of North Carolina, Chapel Hill
- Beth Cimini, Broad Institute
- Daniel Cortes, Virginia Tech
- Benedict Diederich, UC2
- Anna-Karin Gustavsson, Rice University
- Gary Laevsky, Princeton University
- Diane Lidke, University of New Mexico
- Paul Maddox, University of North Carolina, Chapel Hill
- Amy Palmer, University of Colorado
- Karsten Bahlmann, Abberior Instruments
- Stephen Ross, Nikon Instruments
- Khalid Salaita, Emory University
- Wendy Salmon, University of North Carolina, Chapel Hill
- Sydney Shaw, Indiana University
- Justin Taraska, NIH
- Simon Watkins, University of Pittsburgh
- Bjorn Paulson, University of Wisconsin