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Nikon Biopipeline SLide scanner

Nikon BioPipeline Slide - High Content Imaging System

The Nikon “BioPipeline” is an automated, widefield slide scanning system equipped with a Marzhauser Slide Express 2 loader with a 120-slide capacity for high-throughput imaging, optics for polarized microscopy, and optics to support UV – IR imaging. Due to the robotic slide loader, the BioPipeline is ideal for high content projects that require imaging several slides in an automated fashion and supports both 1x3 and 2x3 glass slides. The BioPipeline is an assisted-use only system and is offered as a service at the MSR. MSR staff with work with users to design an imaging workflow and acquire the images based on the user’s needs. Researchers interested in this service, supported slides, and turnaround time can view our Slide Scanning Request Form on iLabs. This whole slide imaging (WSI) device has:

  • 2x/0.1, 4x/0.2, 10x/0.45, 20x/0.75, 40x/0.95 Nikon CFI Plan Apo Lambda dry objectives
  • A color camera (Nikon DS-FI3) for brightfield (transmitted light) imaging of stained microscope slides prepared with routine histology and immunohistochemical stains.
  • Optics for polarized light imaging of stains such as picrosirious red.
  • A 16bit greyscale (Hamamatsu ORCA Flash 4.0 v3) camera for multicolor fluorescence imaging. Emission fIlters for fluorescence dyes such as DAPI, CFP, GFP/FITC, YFP. CY3, mCherry, CY5, and CY7 (note - due to significant spectral overlap, it is highly unlikely that any more than 5 channels could be acquired/slide). To assist you, see: https://www.fpbase.org/microscope/wwUUo5S7igkNu6DpxdLJXE/
  • In addition to the standard 1x3 inch glass microscope slides, this scanner can also image the less common 2x3 inch glass microscope slides.
  • An off-line workstation for image analysis (Nikon software) and a 200TB MS Windows file server were part of this purchase.