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Workshop in Advanced Fluorescence Imaging and Dynamics (Irvine, CA) Oct 21 - 24, 2024

The Optical Biology Core and the Laboratory for Fluorescence Dynamics are excited to invite you to participate in our 18th Annual Hands-on Workshop in Advanced Fluorescence Imaging and Dynamics, October 21 - 24, 2024, at the University of California Irvine in beautiful southern California.

Application site: https://www.lfd.uci.edu/workshop/registration/
Fee payment site: https://secure.touchnet.net/C21570_ustores/web/store_main.jsp?STOREID=129
Financial support: Applicants in need of financial support to cover the registration fees may be eligible to apply for support through BioImaging North America (BINA). (Select this option in the registration site)

This 4-day course is limited to ~40 participants to ensure a truly interactive, hands-on experience. The program features a combination of interconnected lectures, hands-on laboratory exercises, demonstrations, and discussions, all aimed at equipping participants with the skills required for advanced microscope data analysis. Participants will receive step-by-step guidance on utilizing confocal imaging systems commonly available in Imaging Core facilities, with expert assistance provided for data analysis. They will learn to identify and address potential artifacts, carry out precise measurements, and analyze images for FLIM using open-source software for phasor analysis, hyperspectral imaging unmixing with phasors, and various image correlation methods (ICS, RICS, FCS, and N&B).

A major focus of the course is on optimizing the use of laser scanning and camera-based imaging systems for effective data quantification. The "experimental lab" will include computational simulation and data analysis training. Additionally, industry mentors will offer instruction on tissue clearing protocols and sample preparation for super-resolution imaging.

***Topics to be covered include:***

  • Advanced fluorescence techniques such as Total Internal Reflection Fluorescence (TIRF), Förster Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy (FRET-FLIM) using phasors, FLIM-phasor analysis, Fluorescence Correlation Spectroscopy (FCS), and Image Correlation Spectroscopy (ICS).
  • Brightness (N&B) analysis for studying aggregation, multispectral or hyperspectral phasor analysis, and super-resolution methods including Localization Microscopy, Stimulated Emission Depletion Microscopy (STED), and Structured Illumination Microscopy (SIM).
  • Techniques in Confocal and Multiphoton Laser Scanning Microscopy, as well
    as Light-Sheet Microscopy.

***How will this workshop enhance your training and/or research?***
This workshop is tailored for research scientists, postdoctoral researchers, core facility directors and staff, and graduate students engaged in the biological sciences. It is also suitable for those developing microscopy imaging systems or open-source software for phasor-based analysis. Participants will have the opportunity to discuss specific research challenges with faculty and mentors, gain insights into new instruments and state-of-the-art equipment, and explore the selection of tools that can further their research discoveries.

Best wishes,
The LFD/OBC workshop organizers
LFD: Prof. Michelle Digman (Chair BME UCI), Dr. Francesco Palombo (LFD Lab Manager) OBC <fpalomba@uci.edu>: Dr.Adeela Syed (Optical Biology Core, UCI, Manager) Adeela <adeelas@uci.edu>