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Tissue Acquisition and Cellular/Molecular Analysis Shared Resource

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Nikon/Crest spinning disk confocal

Nikon/Crest spinning disk confocal 

The instrument is a Nikon Ti2-E inverted microscope with a Crest X-Light V2 L-FOV Spinning Disk Confocal and a Photometrics Prime 95B-25MM Back-illuminated sCMOS Camera. The instrument includes multiple laser lines and is well equipped for live cell or organoid imaging.

  • Objective lenses: Plan Fluor 4x/0.13, 10x/0.3; Plan Apochromat Lambda 20x/0.75, 40x/0.95, 60x/1.4 (oil)
  • Laser wavelengths: 405, 445, 473, 520, 545, 635nm
  • Fluorescence emission filters: 438/24, 485/20, 511/20, 560/25, 595/31, 685/40
  • To examine how your fluorescent labels would work with the lasers and filters, see: https://www.fpbase.org/microscope/NSt3svJtnMmkQL7aC69Hb3/
  • Additional brightfield color camera (Nikon DS-F13) and DIC (20x, 40x, 60x), Phase contrast (4x, 10x) imaging modes
  • Stagetop incubation chamber for long term imaging, with the capability of performing hypoxia studies.
  • Multiple Nikon software modules for multi-dimensional imaging, object tracking, image analysis, and deconvolution
Capabilities: