A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

Nikon AX R Confocal Microscope

Nikon AX R Confocal Microscope

The Nikon AX R is an inverted laser-scanning confocal microscope and is our most capable instrument. The AX R has both a traditional, high resolution Galvano scanner and a highspeed Resonant scanner with up to 2K X 2K resolution, making this instrument capable of both high resolution and the largest selection of laser lines (405/445/488/568/594/640/730), allowing the microscope to excite up to 7 fluorophores with clear spectral separation. This instrument has the widest range of objectives lenses, including a long working distance 20x 0.7NA objective with 2.3mm of working distance and water immersion 40x 1.25NA objective with automated water immersion dispenser, ideal for long-term, live cell and multi-well plate imaging.

  • Confocal imaging with high speed Resonant scanner
  • Live cell imaging (humidity, temperature, and CO)
  • Advanced multichannel imaging (up to 7 fluorophores per sample)
  • Long-working 20x objective with 2.3mm working distance
  • Water-immersion 40x objective with automated water dispenser for multiwell imaging

Microscopy Shared Resource

The Microscopy Shared Resource (MSR) is an advanced microscopy facility located at the UA Cancer Center, servicing the immediate needs and future demands of biomedical and translational researchers.

The MSR provides several advanced instruments and services for all UA researchers, including confocal microscopy, super resolution microscopy, slide scanning, live cell imaging, multiphoton and intravital imaging, advanced image analysis, and much more. In addition to providing advanced instrumentation and imaging services, the core facility is a model of academic collaboration. MSR provides a space for on-site workshops, imaging seminars highlighting cutting-edge imaging approaches, demonstrations of next components/instruments, and support to the broader imaging community by building an “imaging culture” on campus.

UA Cancer Center, rooms 0930 (Levy building), 0960 (Salmon building)

Contact the Shared Resource for acknowledgement text. As an NIH P30 center, it is crucial that users of this Shared Resource acknowledge the support of the Cancer Center Support Grant (P30 CA023074).

Core Staff
Ghassan (Gus) Mouneimne, PhD Director, UACC Microscopy Shared Resource 520-626-4616 gmouneimne@arizona.edu
Marco Padilla-Rodriguez, PhD Manager, UACC Microscopy Shared Resource 520-626-6041 marco@arizona.edu
David Jones Jr Staff, UACC Microscopy Shared Resource djonesjr@arizona.edu
University of Arizona Cancer Center wordmark

This facility is administered by the University of Arizona Cancer Center (NCI P30 CA023074), Tucson, Arizona.

Zeiss Axio Observer 7 with Apotome III Microscope

Zeiss Axio Observer 7 with Apotome III Microscope 

This is a multi-function, motorized inverted microscope that includes a brightfield color camera and a 14bit greyscale fluorescence camera. The addition of the Apotome III attachment allows this microscope to give crisp "confocal-like" z-stack images of thinner samples. Thinner means out to approximately 20um in thickness, making this microscope suitable for samples such as: cells on a coverslip, paraffin and standard cryostat sections.

How does this microscope create "confocal-like" images? - The Apotome III projects a grid structure into the focal plane of the specimen, then moves it into different positions using a scanning mechanism. At each grid position, the Apotome III automatically acquires a digital image. The system processes all images into one optical section with improved contrast and increased resolution using a patented algorithm. The resulting image is free from grid structures. (SOURCE: Zeiss) Deconvolution software is available to further improve the image quality of the Apotome images.


  • AI sample finder - using a unique contrasting method, the microscope can find multiple wells in a plate, unstained sections on a slide, or locate the edges of a coverslip . Very useful for setting things up to scan multiple areas or large area scanning of tissue that's otherwise difficult to see by eye.
  • Colibri 7 LED illuminator allows for 7 specific excitation wavelengths (including a near-IR wavelength). With just two multi-band filter sets, switching between channels is significantly faster than microscopes using filter cubes, making tile scanning in fluorescence much faster. Due to spectral overlap issues, we should be able to capture up to 5 channels in regular widefield fluorescence and 4 channels with the Apotome III (which does not work with near-IR wavelengths). To see how your dyes might work with this system, see our configuration at FPbase.
  • Capable of color brightfield imaging (e.g., histology stained slides), Differential Interference Contrast (DIC, e.g., unstained cells), simple Polarized light imaging (crossed linear polarizers, e.g., Picrosirious red staining), and multiple channels of fluorescence as well as combinations of any of these imaging modes.


  • Objective lenses: 5x/0.16NA dry, 10x/0.45NA dry, 20x/0.8NA dry, 40x/1.3NA oil, 63x/1.4NA oil (a 40x/0.95NA dry objective with coverslip correction is available upon advance request)
  • Colibri 7 excitation wavelengths: UV 385/30nm, V 423/44nm, B 469/38nm, G 555/30nm, Y 590/27nm, R 631/33nm, FR 735/40nm
  • Zeiss 489112-9110-000 (suitable for fluorescent dyes like DAPI, FITC, DsRed, CY5, and CY7) and 489091-9110-000 (suitable for fluorescent dyes like CFP, YFP, and mCherry) multiband filter sets

NOTE: See our news announcement Introducing the Zeiss Axio Observer 7 inverted microscope with Apotome III for a discussion of what types of samples would benefit from this microscope, as well as a list of weaknesses.

Nikon/Crest spinning disk confocal

Nikon/Crest spinning disk confocal

Nikon Crest X-Light V2 Spinning-Disk Confocal

The Nikon “Crest” system is an inverted, spinning-disk confocal microscope that allows for high-throughput, confocal imaging of cell and tissue samples. The Crest Spinning-Disk is ideal for applications that require the increased resolution and contrast of confocal imaging with the speed of a widefield system. The Crest Spinning-Disk is equipped with a stage-top environmental incubator for live cell imaging and backilluminated sCMOS Prime95B camera, making it ideal for imaging living samples (cells, organoids, etc.) with minimal phototoxicity.

  • Objective lenses: Plan Fluor 4x/0.13, 10x/0.3; Plan Apochromat Lambda 20x/0.75, 40x/0.95, 60x/1.4 (oil)
  • Laser wavelengths: 405, 445, 473, 520, 545, 635nm
  • Fluorescence emission filters: 438/24, 485/20, 511/20, 560/25, 595/31, 685/40
  • To examine how your fluorescent labels would work with the lasers and filters, see: https://www.fpbase.org/microscope/NSt3svJtnMmkQL7aC69Hb3/
  • Additional brightfield color camera (Nikon DS-F13) and DIC (20x, 40x, 60x), Phase contrast (4x, 10x) imaging modes
  • Stagetop incubation chamber for long term imaging, with the capability of performing hypoxia studies.
  • CFY/YFP FRET imaging
  • Multiple Nikon software modules for multi-dimensional imaging, object tracking, image analysis, and deconvolution

DeltaVision RT Deconvolution Microscope

Deltavision microscope

The DeltaVision RT Deconvolution Microscopy System includes a research-grade inverted Olympus IX 70 microscope (with objectives from 4x to 100x, a cooled CCD camera, fiber-optic coupled mercury arc lamp, high-speed filter changers, electronic shutters and a high precision x-y-z positioning system) that is coupled to a computer workstation running Linux and vendor-supplied software called SoftWorX. Automated multiple wavelength (Blue, Green, Red and Far-red) and/or time lapse imaging experiments can be performed on either fixed tissues or cultured cells (Temperature and gas-mix can be controlled). The Microscope is capable of high resolution fluorescence images, 2D and 3D time-lapse, lower light intensity (compared to a confocal laser) means it is friendlier to live cells, has a greater dynamic range (4096 intensity levels) compared to older confocals, up to 4 probes per data set (limited to the available filter sets).

Training: Users must complete a minimum of 3 hours of training time before they will be allowed to use the instrument without assistance.

Fees: Trained users will be charged a fee of $22.00/hour for time on the instrument (microscope use and/or computation are currently the same fee). Training or Asssisted use are $38.00/hour (NOTE: these rates are internal UA only, updated Feb 2021).

Research Microscopy Core Service

Featuring a DeltaVision RT Deconvolution Fluorescence Microscope with full environmental controls.

Core Staff
Chris Pappas, PhD Director, Research Microscopy Core Service (College of Medicine) 520-626-5209 ctpappas@arizona.edu
Cellular & Molecular Medicine

This facility is administered by the Cellular & Molecular Medicine department of the College of Medicine at the University of Arizona.

Deltavision Elite deconvolution fluorescence microscope

MCB Deltavision microscope

Inverted deconvolution fluorescence microscope with high precision XYZ motorized stage/focus control and an environmental enclosure for live cell imaging. Includes 10x-100x objectives, with two filter sets for fluorescent proteins and standard immuno labels. The motorized stage is equipped to image 96-well plates.

MCB Imaging facility

Light microscopy including deconvolution fluorescence, brightfield imaging with color and greyscale cameras, image analysis software.

Core Staff
Ross Buchan, PhD Director, Dept. Molecular & Cellular Biology Imaging Facility 520-971-7633 rbuchan@arizona.edu
Molecular & Cellualr Biology

This facility is administered by the Molecular and Cellular Biology department in the College of Science at the University of Arizona.

Zeiss LSM 710 Confocal

Zeiss LSM710 confocal

The LSM 710 is an inverted confocal microscope equipped to handle all your fixed tissue imaging needs. A confocal uses point illumination to help overcome some of the limitations of traditional wide-field fluorescence microscopes, including improved optical resolution at depth, decreased background fluorescence and thin optical sectionining for 3D reconstructions.  It can collect transmitted light images (bright field and DIC), as well as conventional and confocal fluorescence images. The microscope is a top of the line spectral point scanner equipped with the following laser lines (405, 458, 488, 514, 561, and 633nm) software for image processing and analysis are also available for use.  

Biomedical Imaging Core

The Biomedical Imaging Core offers leading edge multiphoton and confocal microscopy for biomedical imaging and data collection. Conveniently located on the downtown Phoenix Biomedical Campus at the UA College of Medicine – Phoenix, the core is designed to be a community-based resource available to UA affiliated and non-affiliated faculty and researchers.

For questions, or to learn more about how the Biomedical Imaging Core can enhance your research, please contact Kurt Gustin

Arizona Biomedical Collaborative (ABC, Phoenix), room 170

Core Staff
Kurt Gustin, PhD Director, Biomedical Imaging Core (College of Medicine - Phoenix) 602-827-2155 kgustin@arizona.edu
Shenfeng Qiu, PhD Director, Biomedical Imaging Core (College of Medicine - Phoenix) 602-827-2173 sqiu@arizona.edu
College of Medicine Phoenix logo

This facility is administered by the College of Medicine - Phoenix, at the University of Arizona.