Nikon AX R Confocal Microscope

The Nikon AX R is an inverted laser-scanning confocal microscope and is our most capable instrument. The AX R has both a traditional, high resolution Galvano scanner and a highspeed Resonant scanner with up to 2K X 2K resolution, making this instrument capable of both high resolution and the largest selection of laser lines (405/445/488/568/594/640/730), allowing the microscope to excite up to 7 fluorophores with clear spectral separation. This instrument has the widest range of objectives lenses, including a long working distance 20x 0.7NA objective with 2.3mm of working distance and water immersion 40x 1.25NA objective with automated water immersion dispenser, ideal for long-term, live cell and multi-well plate imaging.

• Confocal imaging with high speed Resonant scanner
• Live cell imaging (humidity, temperature, and CO)
• Advanced multichannel imaging (up to 7 fluorophores per sample)
• Long-working 20x objective with 2.3mm working distance
• Water-immersion 40x objective with automated water dispenser for multiwell imaging

The Microscopy Shared Resource (MSR) is an advanced microscopy facility located at the UA Cancer Center, servicing the immediate needs and future demands of biomedical and translational researchers.

The MSR provides several advanced instruments and services for all UA researchers, including confocal microscopy, super resolution microscopy, slide scanning, live cell imaging, multiphoton and intravital imaging, advanced image analysis, and much more. In addition to providing advanced instrumentation and imaging services, the core facility is a model of academic collaboration. MSR provides a space for on-site workshops, imaging seminars highlighting cutting-edge imaging approaches, demonstrations of next components/instruments, and support to the broader imaging community by building an “imaging culture” on campus.

Zeiss Axio Observer 7 with Apotome III Microscope 

This is a multi-function, motorized inverted microscope that includes a brightfield color camera and a 14bit greyscale fluorescence camera. The addition of the Apotome III attachment allows this microscope to give crisp "confocal-like" z-stack images of thinner samples. Thinner means out to approximately 20um in thickness, making this microscope suitable for samples such as: cells on a coverslip, paraffin and standard cryostat sections.

How does this microscope create "confocal-like" images? - The Apotome III projects a grid structure into the focal plane of the specimen, then moves it into different positions using a scanning mechanism. At each grid position, the Apotome III automatically acquires a digital image. The system processes all images into one optical section with improved contrast and increased resolution using a patented algorithm. The resulting image is free from grid structures. (SOURCE: Zeiss) Deconvolution software is available to further improve the image quality of the Apotome images.

Features:

• AI sample finder - using a unique contrasting method, the microscope can find multiple wells in a plate, unstained sections on a slide, or locate the edges of a coverslip . Very useful for setting things up to scan multiple areas or large area scanning of tissue that's otherwise difficult to see by eye.
• Colibri 7 LED illuminator allows for 7 specific excitation wavelengths (including a near-IR wavelength). With just two multi-band filter sets, switching between channels is significantly faster than microscopes using filter cubes, making tile scanning in fluorescence much faster. Due to spectral overlap issues, we should be able to capture up to 5 channels in regular widefield fluorescence and 4 channels with the Apotome III (which does not work with near-IR wavelengths). To see how your dyes might work with this system, see our configuration at FPbase.
• Capable of color brightfield imaging (e.g., histology stained slides), Differential Interference Contrast (DIC, e.g., unstained cells), simple Polarized light imaging (crossed linear polarizers, e.g., Picrosirious red staining), and multiple channels of fluorescence as well as combinations of any of these imaging modes.

Specifications

• Objective lenses: 5x/0.16NA dry, 10x/0.45NA dry, 20x/0.8NA dry, 40x/1.3NA oil, 63x/1.4NA oil (a 40x/0.95NA dry objective with coverslip correction is available upon advance request)
• Colibri 7 excitation wavelengths: UV 385/30nm, V 423/44nm, B 469/38nm, G 555/30nm, Y 590/27nm, R 631/33nm, FR 735/40nm
• Zeiss 489112-9110-000 (suitable for fluorescent dyes like DAPI, FITC, DsRed, CY5, and CY7) and 489091-9110-000 (suitable for fluorescent dyes like CFP, YFP, and mCherry) multiband filter sets

NOTE: See our news announcement Introducing the Zeiss Axio Observer 7 inverted microscope with Apotome III for a discussion of what types of samples would benefit from this microscope, as well as a list of weaknesses.

Advanced Intravital Confocal/Multiphoton Microscope (TBIR)

• Two Photon Excitation 690-1080nm with Chamelon Ultra 2
• Second Harmonic Generation
• Labelfree imaging, green fluorescent protein, red fluorescent protein
• Large motorized stage
• Intravital imaging of small animals and of samples in stage top incubator 

The Translational Bioimaging Resource (TBIR) is housed in the basement of the Biosciences Research Labs (BSRL) building. The 12,000 sq. ft. TBIR serves as a university-wide resource for pre-clinical biomedical imaging. The resource has the capability to image small biological constructs, small and large animals, and humans. For imaging small biological constructs and small animals the TBIR includes intra vital microscopy, MRI, ultrasound, CT, PET, SPECT, and bioluminescence. For imaging larger animals and humans the resource includes MRI and ultrasound. The resource includes personnel to help you with project development and to help you use the equipment.

Image

Nikon Crest X-Light V2 Spinning-Disk Confocal

The Nikon “Crest” system is an inverted, spinning-disk confocal microscope that allows for high-throughput, confocal imaging of cell and tissue samples. The Crest Spinning-Disk is ideal for applications that require the increased resolution and contrast of confocal imaging with the speed of a widefield system. The Crest Spinning-Disk is equipped with a stage-top environmental incubator for live cell imaging and backilluminated sCMOS Prime95B camera, making it ideal for imaging living samples (cells, organoids, etc.) with minimal phototoxicity.

• Objective lenses: Plan Fluor 4x/0.13, 10x/0.3; Plan Apochromat Lambda 20x/0.75, 40x/0.95, 60x/1.4 (oil)
• Laser wavelengths: 405, 445, 473, 520, 545, 635nm
• Fluorescence emission filters: 438/24, 485/20, 511/20, 560/25, 595/31, 685/40
• To examine how your fluorescent labels would work with the lasers and filters, see: https://www.fpbase.org/microscope/NSt3svJtnMmkQL7aC69Hb3/
• Additional brightfield color camera (Nikon DS-F13) and DIC (20x, 40x, 60x), Phase contrast (4x, 10x) imaging modes
• Stagetop incubation chamber for long term imaging, with the capability of performing hypoxia studies.
• CFY/YFP FRET imaging
• Multiple Nikon software modules for multi-dimensional imaging, object tracking, image analysis, and deconvolution

The DeltaVision RT Deconvolution Microscopy System includes a research-grade inverted Olympus IX 70 microscope (with objectives from 4x to 100x, a cooled CCD camera, fiber-optic coupled mercury arc lamp, high-speed filter changers, electronic shutters and a high precision x-y-z positioning system) that is coupled to a computer workstation running Linux and vendor-supplied software called SoftWorX. Automated multiple wavelength (Blue, Green, Red and Far-red) and/or time lapse imaging experiments can be performed on either fixed tissues or cultured cells (Temperature and gas-mix can be controlled). The Microscope is capable of high resolution fluorescence images, 2D and 3D time-lapse, lower light intensity (compared to a confocal laser) means it is friendlier to live cells, has a greater dynamic range (4096 intensity levels) compared to older confocals, up to 4 probes per data set (limited to the available filter sets).

Training: Users must complete a minimum of 3 hours of training time before they will be allowed to use the instrument without assistance.

Fees: Trained users will be charged a fee of $22.00/hour for time on the instrument (microscope use and/or computation are currently the same fee). Training or Asssisted use are $38.00/hour (NOTE: these rates are internal UA only, updated Feb 2021).

Featuring a DeltaVision RT Deconvolution Fluorescence Microscope with full environmental controls.

Image

Inverted deconvolution fluorescence microscope with high precision XYZ motorized stage/focus control and an environmental enclosure for live cell imaging. Includes 10x-100x objectives, with two filter sets for fluorescent proteins and standard immuno labels. The motorized stage is equipped to image 96-well plates.

Light microscopy including deconvolution fluorescence, brightfield imaging with color and greyscale cameras, image analysis software.