FLIM & FRET Microscopy and Label-free NAD(P)H/FAD Metabolic Imaging - Mar 2023 (Univ. of Virginia)

The W.M. Keck Center for Cellular Imaging, a University of Virginia Imaging Center is sponsoring an advanced course on fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging (FLIM). This year, an additional element will be added: label-free NAD(P)H/FAD metabolic FLIM imaging, which the Center has been publishing several papers on. The focus will be on the fluorescence lifetime redox ratio (FLIRR), a novel metric to investigate both, mitochondrial OXPHOS and cytosolic glycolysis, accompanied by a quantitative analysis package which attendees will be trained on at the workshop.

The workshop will be an intensive lecture/laboratory series that will enable participants to develop theoretical and hands-on expertise with various FRET/FLIM/FLIRR microscopy techniques. Attendees will closely interact with the teaching faculty and assistants while using modern research grade microscopes equipped with essential filters, objective lenses, high-sensitivity detectors (PMTs), and state-of-the-art user-friendly software. These 5 days intensive course includes morning lectures and afternoon hands on training on FRET/FLIM/FLIRR microscopy systems. Experienced staff from universities and industry will provide instruction. With a maximum enrollment of 25students, there will be ample opportunity for attendees to work with various state-of-the-art microscopes, and image processing software.

The Histochemical society is supporting TWO fellowships (each $1000) to attend the workshop which you can use it towards the workshop tuition fees.

Website - https://kcci.virginia.edu/workshop-2023
Contact - Dr. Ammasi Periasamy, Director and founder of WM Keck Center for Cellular Imaging, ap3t@virginia.edu
Dates - March 6-11, 2023