A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

Nikon N-STORM

Nikon N-STORM Super Resolution Microscope

Nikon N-Storm

The Nikon “Storm” system is our super resolution microscope, capable of achieving 20nm XY and 50nm Z resolution (10x increase in resolution than a widefield fluorescence microscope). STochastic Optical Reconstruction Microscopy (STORM) is our most advanced imaging modality offered at the MSR and requires a consultation with our MSR team prior to experimentation and sample preparation. This instrument is also capable of acquiring 3D STORM images and is currently optimized to image two fluorescence channels (488nm and 647nm).

Due to the nature of this advanced technique, the Storm system is assisted-use only and the MSR will provide optimized protocols and imaging buffers for users.

Featured Capabilities:

  • Super resolution imaging (10x resolution)
  • 3D STORM (with astigmatism lens)
  • Optimized for 2-channel STORM imaging (488nm and 647nm)
Capabilities:

TACMASR combined microscopes and other equipment lists

Microscopes

Equipment Capabilities Building
Nikon BioPipeline SLIDE Brightfield, Fluorescence, Widefield, Polarized, Microscope slide scanning, Upright optical microscope, Greyscale camera, RGB color camera UA Cancer Center
Nikon N-STORM Fluorescence, Superresolution, Single Molecule Localization Microscopy (PALM/STORM), Upright optical microscope, Greyscale camera UA Cancer Center
Nikon/Crest spinning disk confocal Brightfield, Fluorescence, Widefield, Confocal (Spinning Disk), Phase Contrast, Deconvolution, Microscope slide scanning, Inverted optical microscope, Environmental Chamber (Live Cell Imaging), Motorized stage, Greyscale camera, RGB color camera UA Cancer Center

Other Equipment

Equipment Capabilities Building
Nikon image processing workstation Image processing workstation UA Cancer Center

Nikon/Crest spinning disk confocal

Nikon/Crest spinning disk confocal

Nikon Crest X-Light V2 Spinning-Disk Confocal

The Nikon “Crest” system is an inverted, spinning-disk confocal microscope that allows for high-throughput, confocal imaging of cell and tissue samples. The Crest Spinning-Disk is ideal for applications that require the increased resolution and contrast of confocal imaging with the speed of a widefield system. The Crest Spinning-Disk is equipped with a stage-top environmental incubator for live cell imaging and backilluminated sCMOS Prime95B camera, making it ideal for imaging living samples (cells, organoids, etc.) with minimal phototoxicity.

  • Objective lenses: Plan Fluor 4x/0.13, 10x/0.3; Plan Apochromat Lambda 20x/0.75, 40x/0.95, 60x/1.4 (oil)
  • Laser wavelengths: 405, 445, 473, 520, 545, 635nm
  • Fluorescence emission filters: 438/24, 485/20, 511/20, 560/25, 595/31, 685/40
  • To examine how your fluorescent labels would work with the lasers and filters, see: https://www.fpbase.org/microscope/NSt3svJtnMmkQL7aC69Hb3/
  • Additional brightfield color camera (Nikon DS-F13) and DIC (20x, 40x, 60x), Phase contrast (4x, 10x) imaging modes
  • Stagetop incubation chamber for long term imaging, with the capability of performing hypoxia studies.
  • CFY/YFP FRET imaging
  • Multiple Nikon software modules for multi-dimensional imaging, object tracking, image analysis, and deconvolution
Capabilities:

Nikon BioPipeline SLIDE

Nikon Biopipeline SLide scanner

Nikon BioPipeline Slide - High Content Imaging System

The Nikon “BioPipeline” is an automated, widefield slide scanning system equipped with a Marzhauser Slide Express 2 loader with a 120-slide capacity for high-throughput imaging, optics for polarized microscopy, and optics to support UV – IR imaging. Due to the robotic slide loader, the BioPipeline is ideal for high content projects that require imaging several slides in an automated fashion and supports both 1x3 and 2x3 glass slides. The BioPipeline is an assisted-use only system and is offered as a service at the MSR. MSR staff with work with users to design an imaging workflow and acquire the images based on the user’s needs. Researchers interested in this service, supported slides, and turnaround time can view our Slide Scanning Request Form on iLabs. This whole slide imaging (WSI) device has:

  • 2x/0.1, 4x/0.2, 10x/0.45, 20x/0.75, 40x/0.95 Nikon CFI Plan Apo Lambda dry objectives
  • A color camera (Nikon DS-FI3) for brightfield (transmitted light) imaging of stained microscope slides prepared with routine histology and immunohistochemical stains.
  • Optics for polarized light imaging of stains such as picrosirious red.
  • A 16bit greyscale (Hamamatsu ORCA Flash 4.0 v3) camera for multicolor fluorescence imaging. Emission fIlters for fluorescence dyes such as DAPI, CFP, GFP/FITC, YFP. CY3, mCherry, CY5, and CY7 (note - due to significant spectral overlap, it is highly unlikely that any more than 5 channels could be acquired/slide). To assist you, see: https://www.fpbase.org/microscope/wwUUo5S7igkNu6DpxdLJXE/
  • In addition to the standard 1x3 inch glass microscope slides, this scanner can also image the less common 2x3 inch glass microscope slides.
  • An off-line workstation for image analysis (Nikon software) and a 200TB MS Windows file server were part of this purchase.
Capabilities:
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