Nikon AX R Confocal Microscope

The Nikon AX R is an inverted laser-scanning confocal microscope and is our most capable instrument. The AX R has both a traditional, high resolution Galvano scanner and a highspeed Resonant scanner with up to 2K X 2K resolution, making this instrument capable of both high resolution and the largest selection of laser lines (405/445/488/568/594/640/730), allowing the microscope to excite up to 7 fluorophores with clear spectral separation. This instrument has the widest range of objectives lenses, including a long working distance 20x 0.7NA objective with 2.3mm of working distance and water immersion 40x 1.25NA objective with automated water immersion dispenser, ideal for long-term, live cell and multi-well plate imaging.

• Confocal imaging with high speed Resonant scanner
• Live cell imaging (humidity, temperature, and CO)
• Advanced multichannel imaging (up to 7 fluorophores per sample)
• Long-working 20x objective with 2.3mm working distance
• Water-immersion 40x objective with automated water dispenser for multiwell imaging

The Microscopy Shared Resource (MSR) is an advanced microscopy facility located at the UA Cancer Center, servicing the immediate needs and future demands of biomedical and translational researchers.

The MSR provides several advanced instruments and services for all UA researchers, including confocal microscopy, super resolution microscopy, slide scanning, live cell imaging, multiphoton and intravital imaging, advanced image analysis, and much more. In addition to providing advanced instrumentation and imaging services, the core facility is a model of academic collaboration. MSR provides a space for on-site workshops, imaging seminars highlighting cutting-edge imaging approaches, demonstrations of next components/instruments, and support to the broader imaging community by building an “imaging culture” on campus.

Image

The LSM 710 is an inverted confocal microscope equipped to handle all your fixed tissue imaging needs. A confocal uses point illumination to help overcome some of the limitations of traditional wide-field fluorescence microscopes, including improved optical resolution at depth, decreased background fluorescence and thin optical sectionining for 3D reconstructions.  It can collect transmitted light images (bright field and DIC), as well as conventional and confocal fluorescence images. The microscope is a top of the line spectral point scanner equipped with the following laser lines (405, 458, 488, 514, 561, and 633nm) software for image processing and analysis are also available for use.  

Image

With the Olympus FVMPE-RS, countless possibilities for deep tissue observation are finally realized. The system delivers unmatched high-speed imaging, essential for capturing the dynamic in vivo response, with fine laser control pinpointing the precise site for optimum excitation efficiency — even deep within the sample. Accompanied by high S/N-ratio imaging and dedicated Olympus multiphoton objectives, efficient detection of scattered fluorescence photons is also ensured. This upright microscope is coupled with a Spectre Physics Insight DS Dual (680-1300nm) laser. In essence, the Olympus FLUOVIEW FVMPE-RS unites high-speed, deep observation capability with multicolor imaging and powerful laser light stimulation, offering a no compromise solution to answer innovative biological research questions. 

The Biomedical Imaging Core offers leading edge multiphoton and confocal microscopy for biomedical imaging and data collection. Conveniently located on the downtown Phoenix Biomedical Campus at the UA College of Medicine – Phoenix, the core is designed to be a community-based resource available to UA affiliated and non-affiliated faculty and researchers.

For questions, or to learn more about how the Biomedical Imaging Core can enhance your research, please contact Kurt Gustin

Image

Zeiss LSM880 NLO upright multiphoton/confocal with Airyscan

The Zeiss LSM 880 NLO upright multiphoton/confocal microscope includes a 34-channel detector for spectral information and additional non-descanned GAsP detectors. The system includes 7 visible spectrum laser lines (405, 458, 488, 514, 561, 633nm) in addition to the software tunable Spectra Physics Mai Tai HP DeepSee laser (690nm-1040nm). The system includes additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.

The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

Our Resources page: http://microscopy.arizona.edu/ic-optical-resources

Image

Zeiss LSM880 inverted confocal microscope 

The Zeiss LSM 880 inverted confocal microscope includes a 34-channel detector for spectral information, and 7 Laser lines (405, 458, 488, 514, 561, 594, 633nm). The system includes additional software modules for performing FRAP and control for the motorized stage toallow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.

The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures, humidity and CO2 capabilities. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

Objective lenses (with matching DIC prisms) include:

• Plan-Apochromat 10x/0.45 (WD=2.0mm)
• Plan-Apochromat 20x/0.8 (WD=0.55mm)
• Plan-Apochromat 40x/1.3 Oil
• Plan-Apochromat 63x/1.40 Oil

Our Resources page: http://microscopy.arizona.edu/ic-optical-resources

The RII Imaging Cores - Optical facility is a scientific core facility with two locations (Marley and Life Sciences North) dedicated to the acquisition of images for research, industrial and clinical applications. We offer training and multi-user access to high quality state-of-the-art technologies and cost effective analytical services.

The Marley location features an inverted point scanning confocal microscope, an upright confocal/multiphoton microscope with superresolution capabilities, a fluorescence stereo microscope and a research quality microwave. The manager for this location is Patty Jansma.

The Life Sciences North location features a dedicated superresolution fluoresence microscope (structured illumination, SIM), an inverted multifunction widefield microscope (color brightfield, polarized light, fluorescence), an inverted multifunction widefield microscope capable of "confocal-like" images in thinner samples (color brightfield, polarized light, fluorescence), and an image analysis workstation. The manager for this location is Doug Cromey.

The core facility provides training and support for users from the University of Arizona and the community on a fee-for-service basis.

Facility Email: opticalimaging@arizona.edu