Quantitative Imaging: From Acquisition to Analysis (Cold Spring Harbor, NY) Mar/Apr 2025

Combining careful image acquisition with rigorous computational analysis allows extraction of quantitative data from light microscopy images that is far more informative and reproducible than what can be seen by eye. This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens, from tissues to cells to single molecules. The course is designed for quantitative cell and molecular biologists, biophysicists and bioengineers.

We provide a thorough treatment of the complete process of quantitative imaging, from the photons emitted from the sample to the extraction of biologically meaningful measurements from digital images. Material is covered in lectures, discussion groups and hands-on quantitative exercises using commercial microscopes and open-source image analysis tools.

Concepts Covered Include:

• Widefield fluorescence microscopy
• Laser scanning and spinning disk confocal microscopy
• CCD, EMCCD & sCMOS cameras
• Total internal reflection fluorescence microscopy (TIRF)
• Light sheet microscopy
• Super-resolution microscopy (structured illumination, STED & localization microscopy)
• Imaging and analyzing ratiometric “biosensors” (including FRET)
• Fluorescent proteins and live sample imaging
• Image processing (filtering, de-noising, corrections, deconvolution)
• Image segmentation
• Quantitative shape and intensity measurements
• Object detection and tracking
• Machine learning
• Designing and troubleshooting quantitative imaging experiments
• ...and more!

Application Deadline: January 31, 2025
Website: https://meetings.cshl.edu/courses.aspx?course=C-QICM