A volunteer and collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

Zeiss LSM880 NLO upright multiphoton/confocal microscope

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Zeiss LSM880 NLO confocal/multiphoton with Airyscan

Zeiss LSM880 NLO upright multiphoton/confocal with Airyscan

The Zeiss LSM 880 NLO upright multiphoton/confocal microscope includes a 34-channel detector for spectral information and additional non-descanned GAsP detectors. The system includes 7 visible spectrum laser lines (405, 458, 488, 514, 561, 633nm) in addition to the software tunable Spectra Physics Mai Tai HP DeepSee laser (690nm-1040nm). The system includes additional software modules for controlled region-of-interest scanning, deconvolution, physiology experiments, performing FRAP and control for the motorized stage to allow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.

The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

Our Resources page: http://microscopy.arizona.edu/ic-optical-resources

Zeiss LSM880 inverted confocal microscope

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Ziess LSM880 inverted confocal microscope

Zeiss LSM880 inverted confocal microscope 

The Zeiss LSM 880 inverted confocal microscope includes a 34-channel detector for spectral information, and 7 Laser lines (405, 458, 488, 514, 561, 594, 633nm). The system includes additional software modules for performing FRAP and control for the motorized stage toallow stitching together images from multiple fields of view into one larger image (montage), as well as visiting multiple sites during a time lapse acquisition.

The instrument includes an environmental chamber suitable for live cell imaging at a variety of temperatures, humidity and CO2 capabilities. The facility has wet lab space, cell culture incubators, and a biosafety hood in an adjacent room.

Objective lenses (with matching DIC prisms) include:

  • Plan-Apochromat 10x/0.45 (WD=2.0mm)
  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • Plan-Apochromat 40x/1.3 Oil
  • Plan-Apochromat 63x/1.40 Oil

Our Resources page: http://microscopy.arizona.edu/ic-optical-resources

Imaging Cores - Optical

The RII Imaging Cores - Optical facility is a scientific core facility with two locations (Marley and Life Sciences North) dedicated to the acquisition of images for research, industrial and clinical applications. We offer training and multi-user access to high quality state-of-the-art technologies and cost effective analytical services.

The Marley location features an inverted point scanning confocal microscope, an upright confocal/multiphoton microscope with superresolution capabilities, a fluorescence stereo microscope and a research quality microwave. The manager for this location is Patty Jansma.

The Life Sciences North location features a dedicated superresolution fluoresence microscope (structured illumination, SIM), an inverted multifunction widefield microscope (color brightfield, polarized light, fluorescence), an inverted multifunction widefield microscope capable of "confocal-like" images in thinner samples (color brightfield, polarized light, fluorescence), and an image analysis workstation. The manager for this location is Doug Cromey.

The core facility provides training and support for users from the University of Arizona and the community on a fee-for-service basis.

Facility Email: opticalimaging@arizona.edu 

Life Sciences North rooms 410 & 429

Marley suite 101

RII Core Facilities policy regarding acknowledging cores and staff - https://live-core-facilities.pantheonsite.io/acknowledging-cores

University of Arizona - UA RII Imaging Cores - Optical core facility, RRID:SCR_012429  RRID is actually AZ Genetics Core

Core Staff
Doug Cromey, MS Co-Manager, RII Imaging Cores - Optical 520-626-2824 dcromey@arizona.edu
Patty Jansma, MS Co-Manager, RII Imaging Cores - Optical 520-621-5097 pjansma@arizona.edu
Research Innovation & Impact - Core Facilities wordmark

This facility is administered by the Core Facilities department of the Office for Research, Innovation and Impact at the University of Arizona.